The customised solution for research and routine multispecies analysis
- Utilises patented, proven and market-leading technology
- Minimal upkeep, small sample volume and unsurpassed flexibility
- Easy-to-use software developed specifically for veterinary haematology
- 10 pre-programmed species and 20 laboratory customisable species
- GLP Complient
With its open and robust design, the XT-iV analyser lets you run unique fluid types, such as bronchoalveolar lavage fluid with ease. Gates can be modified on previously run sample results without rerunning the specimen
- Patented fluorescence flow cytometry technology
- Easy to use open software design
- Unlimited profiles stored within each species
- Manual gating for a variety of applications
- Whole blood and body fluids
- Small aspiration volume
- Minimal maintenance
Red Blood Cells (RBCs) and Platelets: RBCs and platelets are counted in a dedicated channel using the direct current detection method with hydrodynamic focusing technology to minimise coincidence or recirculation. Automatic discriminators separate the two cell populations based on complex algorithms. The electronic pulse height generated by each analysed RBC is proportional to the cell volume. The haematocrit (HCT) is directly determined based on the count and volume detection of each individual RBC. Even with samples at extremely low or unusually high concentrations, the Sysmex cell counters analyse RBCs and platelets with uncompromised precision and accuracy.
Haemoglobin analysis: The Sysmex Sodium Lauryl Sulfate (SLS) method for haemoglobin analysis is a noncyanide method. Haemoglobin is determined in a separate channel, minimising interference from high leukocyte concentrations. The SLS method shows excellent correlation with the reference method. With very short reaction times, this method is ideal for high throughput analysers.
Fluorescence flow cytometry: Traditional flow cytometry, considered the best method for the differentiation of cell populations, requires the use of costly antigen-antibody reagents. In addition, the procedures associated with it can be cumbersome and time consuming. Sysmex has refined basic techniques of flow cytometry using highly specific polymethine dyes to adapt this sophisticated technology to the high volume and automation requirements of the clinical laboratory. In order to meet the demands for more accurate, routine differentiation of normal and abnormal cells, flow applications using simple but sophisticated fluorescent dyes will continue to be expanded to fit the needs of routine haematology testing.
WBC differential: Fluorescent labeling is a milestone for the routine WBC differential. Fluorescence measurement reveals the nucleus-plasma ratio of each individually stained cell, enabling the Sysmex XT-iV Series analysers to differentiate five reportable WBC populations. The combination of side scatter (inner complexity of the cell), forward scatter (volume) and fluorescence of nucleic acid material determines the classification of each WBC. The XT-iV Series analysers utilise an Adaptive Cluster Analysis System (ACAS) to separate cell populations into well-defined clusters by three-dimensional measurement of WBC providing an accurate and precise differential.
Reticulocytes and platelets: Fluorescence technology is also used to offer efficient and reproducible analysis of the reticulocyte count and maturation stages. The advanced technology allows an accurate count of reticulocytes and fluorescent platelets, even in low concentrations and in samples with giant platelets or red cell fragments. Due to higher fluorescence intensity of nucleated cells, such as leukocytes and nucleated red blood cells, these cells are distinctly separated from reticulocytes. The RNA content of reticulocytes decreases over time until they mature into erythrocytes that are without nucleic acid residues. A population of very early reticulocytes, the Immature Reticulocyte Fraction (IRF) is analysed and reported, providing information about the rate of production of reticulocytes. In addition, measurement of the fluorescence optical platelets (PLT-O) can help resolve impedance platelet flags and reduce manual slide review.
Leading edge technology, customisable software and sample flexibility
Fluorescence flow cytometry: WBC, DIFF, RET, PLT-O
DC sheath flow method: RBC, HCT, PLT-I
Cyanide-free SLS method: HGB
Open design allows for manual gating and user-defined programming of analysis profiles
WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT (PLT-I or PLT-O), NEUT (%, #), LYMPH (%, #), MONO (%, #), EO (%, #), BASO (%, #) RDW-SD, RDW-CV, MPV, P-LCR, PDW, PCT, RET (%, #), LFR, MFR, HFR, IRF
Monkey, rat, mouse, dog, cat, rabbit, guinea pig, cow, pig, horse, additional 20 customisable species
Unlimited in any species and specimens other than blood
150 μL for automated sampler mode
85 μL for manual open mode
40 μL sample volume using capillary mode
Whole blood and body fluids; e.g., bronchoalveolar lavage fluids, bone marrow and body cavity fluids
3000 samples (incl. graphics); 1000 selective test orders
21 QC files, daily external QC IQAS online (optional)
Host (Ethernet or serial)
Graphic printer (parallel or USB)
Auto sampler including internal barcode reader, handheld barcode reader
Main unit with sampler
w x h x d [cm]/[kg]
53 x 63 x 72/59
28 x 40 x 35.5/17
30.5 x 8.5 x 34.5/8
w x h x d [in]/[lbs]
20.9 x 24.8 x 28.3/129.8
11.2 x 14 x 15.8/35.3
12 x 3.3 x 13.6/17.6
One daily automated walk-away shutdown of 15 minutes
*The number of reported parameters may vary according to species.
Easy-to-use, open software concept provides the ultimate in flexibility
- Modify results using the manual gating tool
- Also useful for running body fluids, such as bronchoalveolar lavages and bone marrow
- Add as many as 20 customised species to the 10 pre-programmed in the system
- Create an unlimited number of analysis profiles for individual species or specimens other than blood
- Apply gating profiles to previously run samples for even further result refinement without rerunning the specimens
- Rely on built-in regulatory compliance
- Complete traceability of all operator actions and comprehensive user administration settings
The following example illustrates the importance of using a specific analytical profile that corresponds optimally to the characteristics of nonhematological samples in terms of high-quality results. If a standard haematology profile is used with a BALF sample from a rat, differentiation of the given cell population is incorrect (Fig. 1). If the same sample is analysed with a specific BALF profile, the results differ markedly (Fig. 2).
Fig. 2: The activated monocytes/macrophages (green population) are correctly counted and displayed when the BALF gate is used. They are located in the upper region of the scattergram. Cells with a significantly elevated nucleic acid content, especially in the cytoplasm, are detected there—usually identifiable from the dark blue staining of their cytoplasm in the smear. This count agrees with the manual count.
Fig. 1: Measuring result of a BALF sample before using a specific BALF gate, resulting in incorrect classification of the cell populations. The 1% monocyte result, in particular, was too low compared with the manual reference.